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cometchip 96-well magnetically sealed cassettes (formers)  (Trevigen)

 
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    Trevigen cometchip 96-well magnetically sealed cassettes (formers)
    Analysis of known DNA damaging agents. ( A ) Etoposide is shown to induce replication-dependent DSBs and cause significant DNA damage at concentrations greater than 1 μM (n = 1069–1374). Shown is the plot of % tail DNA for TK6 and Jurkat cells following exposure (60 min) to the agent at the doses indicated. ( B ) H 2 O 2 did not cause DNA damage below 1 μM, with elevated levels of DNA damage observed at 50 μM (n = 450–1200). Shown is the plot of % tail DNA for TK6 and Jurkat cells following exposure (60 min) to the agent at the doses indicated. ( C ) MNNG, a potent SN1 DNA alkylating agent , was the most genotoxic of the compounds tested and caused the maximum amount of DNA damage that could be measured at 10 μM (n = 1075–1374). Shown is the plot of % tail DNA for TK6 and Jurkat cells following exposure (60 min) to the agent at the doses indicated. ( D ) MMS, an SN2 DNA alkylating agent , induced only modest DNA damage on the cells tested, with significant DNA damage observed only at concentrations above 50 μM (n = 812–1294). All assays were conducted in duplicate. Error bars represent mean ± 95% CI. Shown is the plot of % tail DNA for TK6 and Jurkat cells following exposure (60 min) to the agent at the doses indicated. For each panel, the images below the plot are representative <t>CometChip</t> images at the doses indicated.
    Cometchip 96 Well Magnetically Sealed Cassettes (Formers), supplied by Trevigen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cometchip+96-well+magnetically+sealed+cassettes+%28formers%29/pmc05807538-474-1-7?v=Trevigen
    Average 90 stars, based on 1 article reviews
    cometchip 96-well magnetically sealed cassettes (formers) - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Next generation high throughput DNA damage detection platform for genotoxic compound screening"

    Article Title: Next generation high throughput DNA damage detection platform for genotoxic compound screening

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-20995-w

    Analysis of known DNA damaging agents. ( A ) Etoposide is shown to induce replication-dependent DSBs and cause significant DNA damage at concentrations greater than 1 μM (n = 1069–1374). Shown is the plot of % tail DNA for TK6 and Jurkat cells following exposure (60 min) to the agent at the doses indicated. ( B ) H 2 O 2 did not cause DNA damage below 1 μM, with elevated levels of DNA damage observed at 50 μM (n = 450–1200). Shown is the plot of % tail DNA for TK6 and Jurkat cells following exposure (60 min) to the agent at the doses indicated. ( C ) MNNG, a potent SN1 DNA alkylating agent , was the most genotoxic of the compounds tested and caused the maximum amount of DNA damage that could be measured at 10 μM (n = 1075–1374). Shown is the plot of % tail DNA for TK6 and Jurkat cells following exposure (60 min) to the agent at the doses indicated. ( D ) MMS, an SN2 DNA alkylating agent , induced only modest DNA damage on the cells tested, with significant DNA damage observed only at concentrations above 50 μM (n = 812–1294). All assays were conducted in duplicate. Error bars represent mean ± 95% CI. Shown is the plot of % tail DNA for TK6 and Jurkat cells following exposure (60 min) to the agent at the doses indicated. For each panel, the images below the plot are representative CometChip images at the doses indicated.
    Figure Legend Snippet: Analysis of known DNA damaging agents. ( A ) Etoposide is shown to induce replication-dependent DSBs and cause significant DNA damage at concentrations greater than 1 μM (n = 1069–1374). Shown is the plot of % tail DNA for TK6 and Jurkat cells following exposure (60 min) to the agent at the doses indicated. ( B ) H 2 O 2 did not cause DNA damage below 1 μM, with elevated levels of DNA damage observed at 50 μM (n = 450–1200). Shown is the plot of % tail DNA for TK6 and Jurkat cells following exposure (60 min) to the agent at the doses indicated. ( C ) MNNG, a potent SN1 DNA alkylating agent , was the most genotoxic of the compounds tested and caused the maximum amount of DNA damage that could be measured at 10 μM (n = 1075–1374). Shown is the plot of % tail DNA for TK6 and Jurkat cells following exposure (60 min) to the agent at the doses indicated. ( D ) MMS, an SN2 DNA alkylating agent , induced only modest DNA damage on the cells tested, with significant DNA damage observed only at concentrations above 50 μM (n = 812–1294). All assays were conducted in duplicate. Error bars represent mean ± 95% CI. Shown is the plot of % tail DNA for TK6 and Jurkat cells following exposure (60 min) to the agent at the doses indicated. For each panel, the images below the plot are representative CometChip images at the doses indicated.

    Techniques Used:

    Design of Next-Generation CometChip hardware and work-flow. Close up of the CometChip showing the agarose surface chemically bound on a glass support. The CometChip is inserted into a Former base. When assembled, the Former allows each of the 96 wells in the CometChip to be treated individually. Each well has, in turn, more than 500 microwells that enable cells to be gravity loaded into the CometChip, the result is that all cells are on a single focal plane. The dedicated electrophoresis system limits the field variability by reducing the distance between the two electrodes. After electrophoresis, the CometChip is stained and images are acquired using an automated image cytometer (Celigo). These images are subsequently analyzed using dedicated CAS optimized for the CometChip high throughput methodology.
    Figure Legend Snippet: Design of Next-Generation CometChip hardware and work-flow. Close up of the CometChip showing the agarose surface chemically bound on a glass support. The CometChip is inserted into a Former base. When assembled, the Former allows each of the 96 wells in the CometChip to be treated individually. Each well has, in turn, more than 500 microwells that enable cells to be gravity loaded into the CometChip, the result is that all cells are on a single focal plane. The dedicated electrophoresis system limits the field variability by reducing the distance between the two electrodes. After electrophoresis, the CometChip is stained and images are acquired using an automated image cytometer (Celigo). These images are subsequently analyzed using dedicated CAS optimized for the CometChip high throughput methodology.

    Techniques Used: Electrophoresis, Staining, Cytometry, High Throughput Screening Assay

    Acquisition and analysis of image data. ( A ) Image acquisition and comet analysis using dedicated CAS. Representative image of a single CometChip well after DNA damage treatment. Image is comprised of 16 individual post-acquisition stitched images. The insert shows the region expanded in ( B ). ( B ) Higher magnification of the insert from ( A ), shows individual wells. The CAS automated analysis software detects and boxes each comet and colors the comet based on the intensity of the signal. ( C ) Two examples of comets post-CAS analysis – ( upper ) negative control comet with endogenous level of DNA damage. The DNA does not migrate significantly due to supercoiling. ( lower ) Example of a comet from a highly-damaged cell that has been exposed to etoposide. In each example, the white line at the bottom of the image marks the beginning of the comet head, the red line marks the outermost edge of the comet head and the green line marks the end of the comet tail.
    Figure Legend Snippet: Acquisition and analysis of image data. ( A ) Image acquisition and comet analysis using dedicated CAS. Representative image of a single CometChip well after DNA damage treatment. Image is comprised of 16 individual post-acquisition stitched images. The insert shows the region expanded in ( B ). ( B ) Higher magnification of the insert from ( A ), shows individual wells. The CAS automated analysis software detects and boxes each comet and colors the comet based on the intensity of the signal. ( C ) Two examples of comets post-CAS analysis – ( upper ) negative control comet with endogenous level of DNA damage. The DNA does not migrate significantly due to supercoiling. ( lower ) Example of a comet from a highly-damaged cell that has been exposed to etoposide. In each example, the white line at the bottom of the image marks the beginning of the comet head, the red line marks the outermost edge of the comet head and the green line marks the end of the comet tail.

    Techniques Used: Software, Negative Control

    CometChip validation. ( A ) Intra-assay variability. The wells on the outer edge of the plate had the most variability compared to controls. Overall, we observed average variability of less than 4.2% of mean difference and maximum variability less than 10% of mean difference. The average group value is depicted as a red dotted line. Error bars = mean with 95% CI (n = 644–749). ( B ) Inter-assay variability. Cells were treated with etoposide (5 μM) and DNA damage was measured after 1 h. Each point represents a separate experiment (n = 17 per cell line). CV was measured at 13% for the JK cells and 11% for the TK6 cells (n = 420–580 per point). Error bars = mean ± SD. Statistical analysis was conducted via a two-sided Student’s t-test. ( C ) Linear DNA damage response to the DSB causing agent, etoposide. Assay was able to resolve differences in DNA damage caused by 1 μM increments of the compound (****p < 0.0001, n = 1000–2000 comets; r = 0.98). Statistical analysis was conducted via a two-sided Student’s t-test.
    Figure Legend Snippet: CometChip validation. ( A ) Intra-assay variability. The wells on the outer edge of the plate had the most variability compared to controls. Overall, we observed average variability of less than 4.2% of mean difference and maximum variability less than 10% of mean difference. The average group value is depicted as a red dotted line. Error bars = mean with 95% CI (n = 644–749). ( B ) Inter-assay variability. Cells were treated with etoposide (5 μM) and DNA damage was measured after 1 h. Each point represents a separate experiment (n = 17 per cell line). CV was measured at 13% for the JK cells and 11% for the TK6 cells (n = 420–580 per point). Error bars = mean ± SD. Statistical analysis was conducted via a two-sided Student’s t-test. ( C ) Linear DNA damage response to the DSB causing agent, etoposide. Assay was able to resolve differences in DNA damage caused by 1 μM increments of the compound (****p < 0.0001, n = 1000–2000 comets; r = 0.98). Statistical analysis was conducted via a two-sided Student’s t-test.

    Techniques Used: Biomarker Discovery, Intra Assay, Inter Assay

    National Toxicology Program 74 compound plate – agents scored as damaging by CometChip (in descending order by mean % tail DNA within each category). (P value was calculated using a one tailed Student’s t-test).
    Figure Legend Snippet: National Toxicology Program 74 compound plate – agents scored as damaging by CometChip (in descending order by mean % tail DNA within each category). (P value was calculated using a one tailed Student’s t-test).

    Techniques Used: Significance Assay, One-tailed Test

    National Toxicology Program 74 compound plate – agents scored as non-damaging by CometChip (in descending order by mean % tail DNA within each category).
    Figure Legend Snippet: National Toxicology Program 74 compound plate – agents scored as non-damaging by CometChip (in descending order by mean % tail DNA within each category).

    Techniques Used:

    27 known genotoxic agents in the NTP plate and results of the CometChip pre-screen (100 µM concentration, 30-min exposure duration).
    Figure Legend Snippet: 27 known genotoxic agents in the NTP plate and results of the CometChip pre-screen (100 µM concentration, 30-min exposure duration).

    Techniques Used: Concentration Assay, In Vitro, Single Cell Gel Electrophoresis



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    cometchip 96-well magnetically sealed cassettes (formers)  (Trevigen)
    90
    Trevigen cometchip 96-well magnetically sealed cassettes (formers)
    Analysis of known DNA damaging agents. ( A ) Etoposide is shown to induce replication-dependent DSBs and cause significant DNA damage at concentrations greater than 1 μM (n = 1069–1374). Shown is the plot of % tail DNA for TK6 and Jurkat cells following exposure (60 min) to the agent at the doses indicated. ( B ) H 2 O 2 did not cause DNA damage below 1 μM, with elevated levels of DNA damage observed at 50 μM (n = 450–1200). Shown is the plot of % tail DNA for TK6 and Jurkat cells following exposure (60 min) to the agent at the doses indicated. ( C ) MNNG, a potent SN1 DNA alkylating agent , was the most genotoxic of the compounds tested and caused the maximum amount of DNA damage that could be measured at 10 μM (n = 1075–1374). Shown is the plot of % tail DNA for TK6 and Jurkat cells following exposure (60 min) to the agent at the doses indicated. ( D ) MMS, an SN2 DNA alkylating agent , induced only modest DNA damage on the cells tested, with significant DNA damage observed only at concentrations above 50 μM (n = 812–1294). All assays were conducted in duplicate. Error bars represent mean ± 95% CI. Shown is the plot of % tail DNA for TK6 and Jurkat cells following exposure (60 min) to the agent at the doses indicated. For each panel, the images below the plot are representative <t>CometChip</t> images at the doses indicated.
    Cometchip 96 Well Magnetically Sealed Cassettes (Formers), supplied by Trevigen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cometchip+96-well+magnetically+sealed+cassettes+%28formers%29/pmc05807538-474-1-7?v=Trevigen
    Average 90 stars, based on 1 article reviews
    cometchip 96-well magnetically sealed cassettes (formers) - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Analysis of known DNA damaging agents. ( A ) Etoposide is shown to induce replication-dependent DSBs and cause significant DNA damage at concentrations greater than 1 μM (n = 1069–1374). Shown is the plot of % tail DNA for TK6 and Jurkat cells following exposure (60 min) to the agent at the doses indicated. ( B ) H 2 O 2 did not cause DNA damage below 1 μM, with elevated levels of DNA damage observed at 50 μM (n = 450–1200). Shown is the plot of % tail DNA for TK6 and Jurkat cells following exposure (60 min) to the agent at the doses indicated. ( C ) MNNG, a potent SN1 DNA alkylating agent , was the most genotoxic of the compounds tested and caused the maximum amount of DNA damage that could be measured at 10 μM (n = 1075–1374). Shown is the plot of % tail DNA for TK6 and Jurkat cells following exposure (60 min) to the agent at the doses indicated. ( D ) MMS, an SN2 DNA alkylating agent , induced only modest DNA damage on the cells tested, with significant DNA damage observed only at concentrations above 50 μM (n = 812–1294). All assays were conducted in duplicate. Error bars represent mean ± 95% CI. Shown is the plot of % tail DNA for TK6 and Jurkat cells following exposure (60 min) to the agent at the doses indicated. For each panel, the images below the plot are representative CometChip images at the doses indicated.

    Journal: Scientific Reports

    Article Title: Next generation high throughput DNA damage detection platform for genotoxic compound screening

    doi: 10.1038/s41598-018-20995-w

    Figure Lengend Snippet: Analysis of known DNA damaging agents. ( A ) Etoposide is shown to induce replication-dependent DSBs and cause significant DNA damage at concentrations greater than 1 μM (n = 1069–1374). Shown is the plot of % tail DNA for TK6 and Jurkat cells following exposure (60 min) to the agent at the doses indicated. ( B ) H 2 O 2 did not cause DNA damage below 1 μM, with elevated levels of DNA damage observed at 50 μM (n = 450–1200). Shown is the plot of % tail DNA for TK6 and Jurkat cells following exposure (60 min) to the agent at the doses indicated. ( C ) MNNG, a potent SN1 DNA alkylating agent , was the most genotoxic of the compounds tested and caused the maximum amount of DNA damage that could be measured at 10 μM (n = 1075–1374). Shown is the plot of % tail DNA for TK6 and Jurkat cells following exposure (60 min) to the agent at the doses indicated. ( D ) MMS, an SN2 DNA alkylating agent , induced only modest DNA damage on the cells tested, with significant DNA damage observed only at concentrations above 50 μM (n = 812–1294). All assays were conducted in duplicate. Error bars represent mean ± 95% CI. Shown is the plot of % tail DNA for TK6 and Jurkat cells following exposure (60 min) to the agent at the doses indicated. For each panel, the images below the plot are representative CometChip images at the doses indicated.

    Article Snippet: The CometChip Platform is now available from Trevigen, a division of Bio-Techne (Minneapolis, MN) and includes the disposable 30 μM CometChips, the CometChip 96-well magnetically sealed cassettes (formers), the CometChip Electrophoresis System (CES) and the Comet Analysis Software (CAS).

    Techniques:

    Design of Next-Generation CometChip hardware and work-flow. Close up of the CometChip showing the agarose surface chemically bound on a glass support. The CometChip is inserted into a Former base. When assembled, the Former allows each of the 96 wells in the CometChip to be treated individually. Each well has, in turn, more than 500 microwells that enable cells to be gravity loaded into the CometChip, the result is that all cells are on a single focal plane. The dedicated electrophoresis system limits the field variability by reducing the distance between the two electrodes. After electrophoresis, the CometChip is stained and images are acquired using an automated image cytometer (Celigo). These images are subsequently analyzed using dedicated CAS optimized for the CometChip high throughput methodology.

    Journal: Scientific Reports

    Article Title: Next generation high throughput DNA damage detection platform for genotoxic compound screening

    doi: 10.1038/s41598-018-20995-w

    Figure Lengend Snippet: Design of Next-Generation CometChip hardware and work-flow. Close up of the CometChip showing the agarose surface chemically bound on a glass support. The CometChip is inserted into a Former base. When assembled, the Former allows each of the 96 wells in the CometChip to be treated individually. Each well has, in turn, more than 500 microwells that enable cells to be gravity loaded into the CometChip, the result is that all cells are on a single focal plane. The dedicated electrophoresis system limits the field variability by reducing the distance between the two electrodes. After electrophoresis, the CometChip is stained and images are acquired using an automated image cytometer (Celigo). These images are subsequently analyzed using dedicated CAS optimized for the CometChip high throughput methodology.

    Article Snippet: The CometChip Platform is now available from Trevigen, a division of Bio-Techne (Minneapolis, MN) and includes the disposable 30 μM CometChips, the CometChip 96-well magnetically sealed cassettes (formers), the CometChip Electrophoresis System (CES) and the Comet Analysis Software (CAS).

    Techniques: Electrophoresis, Staining, Cytometry, High Throughput Screening Assay

    Acquisition and analysis of image data. ( A ) Image acquisition and comet analysis using dedicated CAS. Representative image of a single CometChip well after DNA damage treatment. Image is comprised of 16 individual post-acquisition stitched images. The insert shows the region expanded in ( B ). ( B ) Higher magnification of the insert from ( A ), shows individual wells. The CAS automated analysis software detects and boxes each comet and colors the comet based on the intensity of the signal. ( C ) Two examples of comets post-CAS analysis – ( upper ) negative control comet with endogenous level of DNA damage. The DNA does not migrate significantly due to supercoiling. ( lower ) Example of a comet from a highly-damaged cell that has been exposed to etoposide. In each example, the white line at the bottom of the image marks the beginning of the comet head, the red line marks the outermost edge of the comet head and the green line marks the end of the comet tail.

    Journal: Scientific Reports

    Article Title: Next generation high throughput DNA damage detection platform for genotoxic compound screening

    doi: 10.1038/s41598-018-20995-w

    Figure Lengend Snippet: Acquisition and analysis of image data. ( A ) Image acquisition and comet analysis using dedicated CAS. Representative image of a single CometChip well after DNA damage treatment. Image is comprised of 16 individual post-acquisition stitched images. The insert shows the region expanded in ( B ). ( B ) Higher magnification of the insert from ( A ), shows individual wells. The CAS automated analysis software detects and boxes each comet and colors the comet based on the intensity of the signal. ( C ) Two examples of comets post-CAS analysis – ( upper ) negative control comet with endogenous level of DNA damage. The DNA does not migrate significantly due to supercoiling. ( lower ) Example of a comet from a highly-damaged cell that has been exposed to etoposide. In each example, the white line at the bottom of the image marks the beginning of the comet head, the red line marks the outermost edge of the comet head and the green line marks the end of the comet tail.

    Article Snippet: The CometChip Platform is now available from Trevigen, a division of Bio-Techne (Minneapolis, MN) and includes the disposable 30 μM CometChips, the CometChip 96-well magnetically sealed cassettes (formers), the CometChip Electrophoresis System (CES) and the Comet Analysis Software (CAS).

    Techniques: Software, Negative Control

    CometChip validation. ( A ) Intra-assay variability. The wells on the outer edge of the plate had the most variability compared to controls. Overall, we observed average variability of less than 4.2% of mean difference and maximum variability less than 10% of mean difference. The average group value is depicted as a red dotted line. Error bars = mean with 95% CI (n = 644–749). ( B ) Inter-assay variability. Cells were treated with etoposide (5 μM) and DNA damage was measured after 1 h. Each point represents a separate experiment (n = 17 per cell line). CV was measured at 13% for the JK cells and 11% for the TK6 cells (n = 420–580 per point). Error bars = mean ± SD. Statistical analysis was conducted via a two-sided Student’s t-test. ( C ) Linear DNA damage response to the DSB causing agent, etoposide. Assay was able to resolve differences in DNA damage caused by 1 μM increments of the compound (****p < 0.0001, n = 1000–2000 comets; r = 0.98). Statistical analysis was conducted via a two-sided Student’s t-test.

    Journal: Scientific Reports

    Article Title: Next generation high throughput DNA damage detection platform for genotoxic compound screening

    doi: 10.1038/s41598-018-20995-w

    Figure Lengend Snippet: CometChip validation. ( A ) Intra-assay variability. The wells on the outer edge of the plate had the most variability compared to controls. Overall, we observed average variability of less than 4.2% of mean difference and maximum variability less than 10% of mean difference. The average group value is depicted as a red dotted line. Error bars = mean with 95% CI (n = 644–749). ( B ) Inter-assay variability. Cells were treated with etoposide (5 μM) and DNA damage was measured after 1 h. Each point represents a separate experiment (n = 17 per cell line). CV was measured at 13% for the JK cells and 11% for the TK6 cells (n = 420–580 per point). Error bars = mean ± SD. Statistical analysis was conducted via a two-sided Student’s t-test. ( C ) Linear DNA damage response to the DSB causing agent, etoposide. Assay was able to resolve differences in DNA damage caused by 1 μM increments of the compound (****p < 0.0001, n = 1000–2000 comets; r = 0.98). Statistical analysis was conducted via a two-sided Student’s t-test.

    Article Snippet: The CometChip Platform is now available from Trevigen, a division of Bio-Techne (Minneapolis, MN) and includes the disposable 30 μM CometChips, the CometChip 96-well magnetically sealed cassettes (formers), the CometChip Electrophoresis System (CES) and the Comet Analysis Software (CAS).

    Techniques: Biomarker Discovery, Intra Assay, Inter Assay

    National Toxicology Program 74 compound plate – agents scored as damaging by CometChip (in descending order by mean % tail DNA within each category). (P value was calculated using a one tailed Student’s t-test).

    Journal: Scientific Reports

    Article Title: Next generation high throughput DNA damage detection platform for genotoxic compound screening

    doi: 10.1038/s41598-018-20995-w

    Figure Lengend Snippet: National Toxicology Program 74 compound plate – agents scored as damaging by CometChip (in descending order by mean % tail DNA within each category). (P value was calculated using a one tailed Student’s t-test).

    Article Snippet: The CometChip Platform is now available from Trevigen, a division of Bio-Techne (Minneapolis, MN) and includes the disposable 30 μM CometChips, the CometChip 96-well magnetically sealed cassettes (formers), the CometChip Electrophoresis System (CES) and the Comet Analysis Software (CAS).

    Techniques: Significance Assay, One-tailed Test

    National Toxicology Program 74 compound plate – agents scored as non-damaging by CometChip (in descending order by mean % tail DNA within each category).

    Journal: Scientific Reports

    Article Title: Next generation high throughput DNA damage detection platform for genotoxic compound screening

    doi: 10.1038/s41598-018-20995-w

    Figure Lengend Snippet: National Toxicology Program 74 compound plate – agents scored as non-damaging by CometChip (in descending order by mean % tail DNA within each category).

    Article Snippet: The CometChip Platform is now available from Trevigen, a division of Bio-Techne (Minneapolis, MN) and includes the disposable 30 μM CometChips, the CometChip 96-well magnetically sealed cassettes (formers), the CometChip Electrophoresis System (CES) and the Comet Analysis Software (CAS).

    Techniques:

    27 known genotoxic agents in the NTP plate and results of the CometChip pre-screen (100 µM concentration, 30-min exposure duration).

    Journal: Scientific Reports

    Article Title: Next generation high throughput DNA damage detection platform for genotoxic compound screening

    doi: 10.1038/s41598-018-20995-w

    Figure Lengend Snippet: 27 known genotoxic agents in the NTP plate and results of the CometChip pre-screen (100 µM concentration, 30-min exposure duration).

    Article Snippet: The CometChip Platform is now available from Trevigen, a division of Bio-Techne (Minneapolis, MN) and includes the disposable 30 μM CometChips, the CometChip 96-well magnetically sealed cassettes (formers), the CometChip Electrophoresis System (CES) and the Comet Analysis Software (CAS).

    Techniques: Concentration Assay, In Vitro, Single Cell Gel Electrophoresis